Expression of HLA-ABC and HLA-DR was observed in 24 fresh surgical specimens of both gastric cancer and adjacent mucosa with flow cytometry after staining with monoclonal antibodies.
In gastric cancer tissues, the HLA-ABC positive cells were 79.9×5.3% and the HLA-DR positive cells were 75.2±5.1%. These ratio of HLA-ABC and HLA-DR positive cells in gastric cancer tissues was higher than those in normal adjacent mucosa
(HLA-ABC;
68.0±6.6%, HLA-DR; 62.0±6.2%). In metastatic gastric cancer, HLA-ABC was higher than normal mucosa in most cases. However, HLA-ABC expression in all cases with peritoneal seeding was less than normal mucosa. In stage I gastric cancers, HLA-ABC
and
HLA-DR positive cells were more than advanced gastric cancers. HLA-ABC positive cells were more common in well and moderately differentiated gastric cancer than in poorly differentiate cancers (85.7%±26.4% : 76.7±27.1%). The HLA-DR positive
cells
were
less in aneuploid cancers than in diploid cancers (70.2±28.1% : 87.8±7.9%).
We established method to quantitative analysis of HLA expresion in tissue. Expression of HLA-ABC & DR were higher in gastric cancer tissues than in normal mucosa. Among the tumors, the HLA-ABC expression was higher in stage I, well differentiated
and
small cancers, and HLA-DR in diploid and nonmetastatic cancers.
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